Two simple, accurate, and reproducible UV spectrophotometric methods for simultaneous estimation of Amoxicillin Trihydrate (AMOX) and Ambroxol Hydrochloride (AMBRO) have been developed in the present work. The first developed method was Q-ratio method, wavelength selected are 228nm (λmax of amoxicillin) and 250nm (iso-absorptive point for both drugs). The second developed method was AUC with wavelength ranges of 223-233 nm and 240-250 nm selected to determine AMOX and AMBRO. Linearity for both the methods was observed in concentration range of 2-12µg/ml for AMBRO and 20-120 µg/ml for AMOX. The correlation coefficients for both the methods obtained were near to 1. The accuracy of these methods were evaluated by recovery studies and good recovery results were obtained from 98 % to 102% for both the methods and the relative standard deviation of below 2% were achieved as per ICH guidelines.
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